495 HEPARIN-BINDING IGF-1 PROVIDES SUSTAINED DELIVERY OF IGF-1 TO CARTILAGE IN VIVO
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چکیده
منابع مشابه
Intra-articular Injection of HB-IGF-1 Sustains Delivery of IGF-1 to Cartilage through Binding to Chondroitin Sulfate
Objective—IGF-1 stimulates cartilage repair but is not a practical therapy due to its short halflife. We have previously modified IGF-1 by adding a heparin-binding domain and have shown that this fusion protein (HB-IGF-1) stimulates sustained proteoglycan synthesis in cartilage. Here, we first examined the mechanism by which HB-IGF-1 is retained in cartilage. We then tested whether HB-IGF-1 pro...
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The vascular insulin-like growth factor (IGF)-1 system includes the IGFs, the IGF-1 receptor (IGF-1R), and multiple binding proteins. This growth factor system exerts multiple physiologic effects on the vasculature through both endocrine and autocrine/paracrine mechanisms. The effects of IGF-1 are mediated principally through the IGF-1R but are modulated by complex interactions with multiple IG...
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Using site-directed mutagenesis, we have undertaken a study of a potential IGF-binding site in the C-terminal domain of rat IGFBP-5, lying close to or within a previously described heparin-binding domain (residues 201-218) in this protein. After analysis of binding activity using three different methods - ligand blotting, solution phase equilibrium binding and biosensor measurement of real-time...
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ژورنال
عنوان ژورنال: Osteoarthritis and Cartilage
سال: 2009
ISSN: 1063-4584
DOI: 10.1016/s1063-4584(09)60516-1